Tatsuya Osaki Postdoctral fellow Email:osaki@mit.eduFocus group: Neuromusccular junction, ALS Department: Mechanical engineering Hometown: Aichi, Japan Undergrad: University of Tsukuba |
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| Research summary: The goal of a project is to develop a complex in vitro neurovascular model including the neuromuscular junction for investigating neurodegenerative diseases. The platform is micro-engineered to include complex interactions between two or more muscle cells, endothelial cells and motor neurons. We grow these cells in 3D in a physiologically relevant extracellular matrix and subject the cells to appropriate mechanical stresses and strains as is found in contractile tissues like muscle tissue. Furthermore, patient derived –motor unit was also established using amyotrophic lateral sclerosis patient derived iPS cells to mimic pathological conditions of ALS. Using this platform, new drug candidate for ALS was screened by testing neurotoxicity. |
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Research achivement: Google schloor click here 【Paper】 Tatsuya Osaki, Uzel Sebastien, Roger D Kamm “Microphysiological 3D model of amyotrophic lateral sclerosis (ALS) from human iPS-derived muscle cells and optogenetic motor neurons”, Science Advances, 4, eaat5847, 2018 Tatsuya Osaki, Jean C. Serrano, Roger D Kamm “Cooperative Effects of Vascular Angiogenesis and Lymphangiogenesis”, Regenerative Engineering and Translational Medicine, 4 (9), 1-13, 2018. Tatsuya Osaki, Vivek Sivathanu, Roger D. Kamm, "Vascularized microfluidic organ-chips for drug screening, disease models and tissue engineering",Current opinion in biotechnology,52, 116-123, 2018 Marco Campisi, Yoojin Shin, Tatsuya Osaki, Cynthia Hajal, V. Chiono, Roger D. Kamm, "3D self-organized microvascular model of the human blood-brain barrier with endothelial cells, pericytes and astrocytes",Biomaterials, 8 (1), 5168, 2018 Tatsuya Osaki, Vivek Sivathanu, Roger D. Kamm, "Engineered 3D vascular and neuronal networks in a microfluidic platform",Scientific Reports, 8 (1), 5168, 2018 Tatsuya Osaki, Vivek Sivathanu, Roger D. Kamm, "Crosstalk between developing vasculature and optogenetically engineered skeletal muscle improves muscle contraction and angiogenesis",Biomaterials, 156, p65-76, 2017. Tatsuya Osaki, Yoojin Shin, Vivek Sivathanu, Marco Campisi, Roger D. Kamm, "In Vitro Microfluidic Models for Neurodegenerative Disorders", Advanced healthcare Materials, 2017. DOI: 10.1002/adhm.201700489 Tatsuya Osaki, Tatsuto Kageyama, Yuka Shimazu, Dina Mysnikova, Shintaro Takahashi, Shinichi Takimoto, Junji Fukuda, "Flatbed epi relief-contrast cellular monitoring system for stable cell culture", Scientific Reports, 7 (1), 2017. Junko Enomoto, Tatsuto Kageyama, Tatsuya Osaki, Flavia Bonalumi, Francesca Marchese, Alfonso Gautieri, Elena Bianchi, Gabriele Dubini, Chiara Arrigoni, Matteo Moretti, Junji Fukuda, "Catch-and-Release of Target Cells Using Aptamer-Conjugated Electroactive Zwitterionic Oligopeptide SAM", Scientific Reports, 7, 2017. Tatsuto Kageyama, Tatsuya Osaki, Junko Enomoto, Dina Myasnikova, Tadashi Nittami, Takuro Hozumi, Taichi Ito, Junji Fukuda, "In Situ Cross-Linkable Gelatin-CMC Hydrogels Designed for Rapid Engineering of Perfusable Vasculatures", ACS, biomaterials and engineering, 2 (6), 2016. Junko Enomoto, Naoto Mochizuki, Katsumi Ebisawa, Tatsuya Osaki, Tatsuto Kageyama, Dina Myasnikova, Tadashi Nittami, Junji Fukuda, "Engineering thick cell sheets by electrochemical desorption of oligopeptides on membrane substrates", Regenerative therapy, 3, 2016. Tatsuya Osaki, Takahiro Kakegawa, Tatsuto Kageyama, Junko Enomoto, Tadashi Nittami, Junji Fukuda, "Acceleration of vascular sprouting from fabricated perfusable vascular-like structures", PLoS One, 10 (4), 2015 Tatsuto Kageyama, Takahiro Kakegawa, Tatsuya Osaki, Junko Enomoto, Taichi Ito, Tadashi Nittami, Junji Fukuda, "Rapid engineering of endothelial cell-lined vascular-like structures in in situ crosslinkable hydrogels", Biofabrication, 6 (2), 2014. Tatsuya Osaki, Takahiro Kakegawa Naoto Mochizuki, Junji Fukuda, " Fabrication of perfusable vasculatures by using micromolding and electrochemical cell transfer ", IEEE EMBC, 6655-6658, 201310.1109/EMBC.2013.6611082 Junji Fukuda, Shintaro Takahashi, Tatsuya Osaki, Naoto Mochizuki and Hiroaki Suzuki, Processing of nanolitre liquid plugs for microfluidic cell-based assays, Sci. Technol. Adv. Master, 13 2012 Naoto Mochizuki, Takahiro Kakegawa, Tatsuya Osaki,Nasser Sadr, Nezamoddin N Kachouie, Hiroaki Suzuki, Junji Fukuda, Tissue engineering based on electrochemical desorption of an RGD-containing oligopeptide, Journal of tissue engineering and regenerative medicine, In press. Junji Fukuda, Shintaro Takahashi, Tatsuya Osaki,Naoto Mochizuki and Hiroaki Suzuki, Processing of nanolitre liquid plugs for microfluidic cell-based assays, Sci. Technol. Adv. Master, 13 2012 Nasser Sadr, Mojun Zhu,Tatsuya Osaki,Takahiro Kakegawa, Yunzhi Yang, Matteo Moretti, Junji Fukuda, Ali Khademhosseini,SAM-based cell transfer to photopatterned hydrogels for microengineering vascular-like structures, Biomaterials 32 (2011) 7479-7490 Tatsuya Osaki, Takahiro kakegawa, Hiroaki Suzuki, Junji Fukuda Electrical Detachment of Cells for engineering Capillary-like structures in a Photocrosslinkable hydrogel,IEEE EMBS, 2451-5254, 2011.
【Conference】 (Oral presentation) （1）Tatsuya Osaki, T. Kakegawa, N. Sadr, A. Khademhosseini, H. Suzuki and J. Fukuda , Electrical Cell Transfer for Engineering Capillary-Like Structures in Photocrosslinkable Hydrogels .Tissue Enginnering and Regenerative Medicine }International Society Asia Pacific(TERMIS-AP) Meeting、Singapore, August, 2011 （2）Tatsuya Osaki, T. Kakegawa, H. Suzuki and J. Fukuda , Electrical Cell Transfer for Engineering Capillary-Like Structures in Photocrosslinkable Hydrogels . SYIS-AP Young Investigator Awards ,Singapore, August, 2011 （3）Tatsuya Osaki, Takahiro Kakegawa, Hiroaki Suzuki, Junji Fukuda, Electrical Detachment of Cells for Engineering Capillary-Like Structures in a Photocrosslinkable Hydrogel, IEEE Engineering in Medicine and Biology Society, Boston, USA, September, 2011 (4) Tatsuya Osaki, Hiroaki Suzuki, Junji Fukuda, Rapid assembly of vascular-like strucutures by electrochemical cell transfer, Manchester, UK, October, 2012 (5)Tatsuya, Osaki.Junji Fukuda, Fabirication of Perfusable Vasculatures Using Micromolding and Electrochemical Cell Transfer, Osaka, Japana, July, 2013 (6)Tatsuya Osaki, Takahiro Kakegawa, Junji Fukuda, Rapid assembly of perfusable microvascular-like structures by electrochemical cell transfer, Termis-America, Atlanta, U.S.A, November, 2014. (7)Tatsuya Osaki, Takanori Takebe, Junji Fukuda, Micro-molding of vascularized liver-like tissues with iPS-derived hepatic endoderm spheroids, ERATO, tokyo, Japan, June, 2014. (8)Tatsuya Osaki, Takanori Takebe, Junji Fukuda, Engineering hepatic tissues with perfusable vascular structures. WC9, Prague, Czech Republic, August, 2014.(Poster) (9)Tatsuya Osaki, Takanori Takebe Junji Fukuda, Fabrication of vascularized liver-like tissues using human iPS hepatic endoderm cells and electrochemical cell transfer, Termis-AP 2014, Daegu, Korea, September 24-27, 2014 (10)Tatsuya Osaki, Takanori Takebe, Junji Fukuda, Micro-Molding of Vascularized Liver-Like Tissues with iPS-Derived Hepatic Endoderm Spheroids, MRS fall meeting 2014, Boston, USA, December 1-5, 2014 (11)Tatsuya Osaki, Takanori Takebe, Junji Fukuda, Engineering of transplantable liver tissues with iPS-derived hepatic spheroids, 2nd world conference of Targeting liver diseases 2015, June25-26, 2015, Winner of the poster award. (12)Tatsuya Osaki, Takahiro Kakegawa, Takanori Takebe, Junji Fukuda, Engineering of liver-like tissues using cell adhesive zwitterionic oligopeptide, 2nd International Conference on Bioinspired and Zwitterionic Materials (ICBZM2015), August 13-14, 2015 (13)Tatsuya Osaki, Hiroaki Suzuki, Junji Fukuda, Enginnering of bio-artificial liver with iPS-derived hepatic cells, Interdiscipinary Workshop on Scienceand Patent 2015(IWP2015), September 4, 2015,Winner of the IWP poster and short oral award.
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